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CellTox Green Cytotoxicity Assay
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CellTox Green Cytotoxicity Assay
CellTox Green Cytotoxicity Assay
CellTox Green Cytotoxicity Assay from Promega
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Citations:
(67)
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CellTox Green Cytotoxicity Assay
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Description
The CellTox Green Cytotoxicity Assay measures changes in membrane integrity that occur as a result of cell death. The assay is intended to assess cytotoxicity in cell culture after experimental manipulation
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
CellTox Green Cytotoxicity Assay
Company
Promega
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
G8741
Quantity
10ml
Reactivity
mammal
Sample Type
cells in culture
Sensitivity
<200 cells="">200>
Time
0-72 hours
Molecular Weight
~61kDa
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Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (67)
(1)
Collective directional migration drives the formation of heteroclonal cancer cell clusters
Molecular Oncology
September 1, 2023
Miriam Palmiero, Isabel Cantarosso, Laura di Blasio, Valentina Monica, Barbara Peracino, Luca Primo, Alberto Puliafito
DAPI. To test the possible cytotoxic effect of the
inhibitors
,
CellTox Green Cytotoxicity Assay
(#
G8741
;
Promega
) was used following manufacturer's instructions. Briefly, after 72 h (24 and 48 h for 2D
assays
)
(2)
Optogenetic Induction of Pyroptosis, Necroptosis, and Apoptosis in Mammalian Cell Lines
BIO-PROTOCOL
July 20, 2023
Kateryna Shkarina, Petr Broz
C4243
)Opti-
MEM
reduced serum medium (
Gibco
, catalog number:
11058021
)CellTox Green (
Promega
, catalog number:
G8741
)DRAQ7 (
BioLegend
, catalog number:
424001
)Annexin V Pacific Blue (BioLegend, catalog number:
640918
)Annexin
(3)
Differential response of C9orf72 transcripts following neuronal depolarization
iScience
June 16, 2023
Layla T. Ghaffari, Davide Trotti, Aaron R. Haeusler
assays
,Critical commercial
assays
,Critical commercial
assays
,,
CellTox™ Green Cytotoxicity Assay
,
Promega
,
G8741
CytoView MEA plate,Axion Biosystems,M384-tMEA-24W CytoView MEA plate,Axion Biosystems,M384-tMEA-24W ,, Deposited
(4)
Well-Defined Heparin Mimetics Can Inhibit Binding of the Trimeric Spike of SARS-CoV-2 in a Length-Dependent Manner
JACS Au
April 24, 2023
Lifeng Sun, Pradeep Chopra, Ilhan Tomris, Roosmarijn van der Woude, Lin Liu, Robert P. de Vries, Geert-Jan Boons
heparin mimetics 1 and 6 was evaluated on Vero E6 cells using the
CellTox green cytotoxicity assay
(
G8741
,
Promega
). It was discovered that compounds 1 and 6 at a concentration of 100 μg/mL exhibit no toxicity,
(5)
Colorectal cancer-derived small extracellular vesicles induce TGFβ1-mediated epithelial to mesenchymal transition of hepatocytes
Cancer cell international
April 18, 2023
Pucci M, Moschetti M, Urzì O, Loria M, Conigliaro A, Di Bella MA, Crescitelli R, Olofsson Bagge R, Gallo A, Santos MF,
et al
.
can have a toxic effect on
hepatocyte
growth, the
CellTox™ Green Cytotoxicity Assay
was performed (
G8741
,
Promega
, Madison, WI, USA). THLE-2 cells were cultured in triplicate at 5 × 103 cells/well in white-walled,
(6)
Protein supersaturation powers innate immune signaling
bioRxiv
March 25, 2023
Alejandro Rodriguez Gama, Tayla Miller, Jeffrey J. Lange, Jianzheng Wu, Xiaoqing Song, Shriram Venkatesan, Jay R. Unruh, Dan Bradford, Randal Halfmann
(
BioVision
,
1544–5
), Puromycin (
Invivogen
,
ant-pr-1
), Sytox
Orange
(
ThermoFisher
,
S11368
), CellTox (
Promega
,
G8741
). Antibodies, anti-FADD (sc-514414), anti-FADD (05–486), anti-Actin (sc-8432). Plasmid construction
Yeast expression
(7)
Evaluating the RIST Molecular-Targeted Regimen in a Three-Dimensional Neuroblastoma Spheroid Cell Culture Model
Cancers (Basel)
March 14, 2023
Carina Kaess, Marie Matthes, Jonas Gross, Rebecca Waetzig, Tilman Heise, Selim Corbacioglu, Gunhild Sommer
calculated by normalizing the measured viability to DMSO (vehicle) controls. CellTox Green Dye (
Promega
, #
G8741
) was applied according to the manufacturer’s instructions. Three replicates were used for each drug
(8)
IDH2 stabilizes HIF-1α-induced metabolic reprogramming and promotes chemoresistance in urothelial cancer
The EMBO journal
February 15, 2023
Shigeta K, Hasegawa M, Hishiki T, Naito Y, Baba Y, Mikami S, Matsumoto K, Mizuno R, Miyajima A, Kikuchi E,
et al
.
Blood & Cell
Culture DNA extraction Kits,
Qiagen
,#Cat:
13323
Cell Tox Green Cytotoxicity Assay,
Promega
,#Cat:
G8741
NADP/NADPH Quantitation Colorimetric Kit
,
Biovision
,#Cat:
K347‐100
CellROX®
Orange
,
Thermo Fischer Scientific
,#Cat:
(9)
Tamoxifen induces radioresistance through NRF2-mediated metabolic reprogramming in breast cancer
Cancer & Metabolism
February 8, 2023
F. V. Naumann, F. C. G. J. Sweep, G. J. Adema, W. J. M. Peeters, J. W. M. Martens, J. Bussink, P. N. Span
measurement Toxicity of H2O2 on
breast cancer
cells were measured with the
CellTOX™ Green cytotoxicity assay
(
G8741
,
Promega
) on the
Incucyte ZOOM
(Essen Bioscience). Cells were seeded and incubated in 96 wells
cell culture
(10)
Interaction between Butyrate and Tumor Necrosis Factor α in Primary Rat Colonocytes
Biomolecules
January 30, 2023
Christopher L. Souders, Juan J. Aristizabal-Henao, Sarah J. Patuel, John A. Bowden, Jasenka Zubcevic, Christopher J. Martyniuk
CellTiter-Glo® (
Promega
, Cat#
G9241
), AlamarBlue™ (Invitrogen, Cat# A50100), CellTox™ Green (
Promega
, Cat#
G8741
), Mitochondrial Membrane Potential Kit (Sigma-Aldrich, Cat# MAK159), and H2DCFDA dye (
Invitrogen
, Cat#
(11)
Hydropersulfides inhibit lipid peroxidation and ferroptosis by scavenging radicals
Nature chemical biology
January 1, 2023
Barayeu U, Schilling D, Eid M, Xavier da Silva TN, Schlicker L, Mitreska N, Zapp C, Gräter F, Miller AK, Kappl R,
et al
.
day, cells were treated with RSL3 or CHP, as indicated in the figure legends. CellTox Green reagent (
G8741
,
Promega
) was added according to the manufacturer’s protocol. Green
fluorescence
(ex 480/em 520) was
(12)
Cancer-targeted photoimmunotherapy induces antitumor immunity and can be augmented by anti-PD-1 therapy for durable anticancer responses in an immunologically active murine tumor model
Cancer Immunology, Immunotherapy
January 1, 2023
Michelle A. Hsu, Stephanie M. Okamura, C. Daniel De Magalhaes Filho, Daniele M. Bergeron, Ahiram Rodriguez, Melissa West, Deepak Yadav, Roger Heim, Jerry J. Fong, Miguel Garcia-Guzman
Spectramax M5 plate reader. Percent cell death was measured using the CellTox Green
assay
(
Promega
, Cat. No.
G8741
) following manufacturer recommendations. Type I interferons and ANXA1 quantification
assays
Cultured
(13)
Combined inhibition of ACLY and CDK4/6 reduces cancer cell growth and invasion
Oncology Reports
December 22, 2022
Brian Christopher Velez, Christopher Paul Petrella, Kathleen Helen DiSalvo, Keyi Cheng, Rebecca Kravtsov, Dorina Krasniqi, Nancy Ann Krucher
apoptosis, additional
Promega
assays
were employed: the
CellTox Green Cytotoxicity Assay
(cat. no.
G8741
), utilized on MDA-MBA-231 cells; and the
RealTime-Glo Annexin V Apoptosis and Necrosis Assay
(cat. no.
(14)
Development and characterization of anti-fibrotic natural compound similars with improved effectivity
Basic Research in Cardiology
December 1, 2022
Fabian Philipp Kreutzer et al.
were treated with serial dilutions of compounds with additional 0.1% CellTox™ Green dye (
Promega
, #
G8741
). After 24 h
incubation
, 5 µL medium was removed from each well, mixed with 95 µL LDH Storage buffer
(15)
A cell-based chemical-genetic screen for amino acid stress response inhibitors reveals torins reverse stress kinase GCN2 signaling
Journal of Biological Chemistry
October 20, 2022
Johanna B. Brüggenthies, Alessandra Fiore, Marion Russier, Christina Bitsina, Julian Brötzmann, Susanne Kordes, Sascha Menninger, Alexander Wolf, Elena Conti, Jan E. Eickhoff,
et al.
confluency
in tissue culture
plates (12-well format) and treated with CellTox Green reagent (
1:4000
,
G8741
,
Promega
) the next day. Cell death was monitored by live-cell
phase-contrast microscopy
over time by
(16)
Anti‐inflammatory properties of lemon‐derived extracellular vesicles are achieved through the inhibition of ERK / NF‐κB signalling pathways
Journal of Cellular and Molecular Medicine
August 1, 2022
Stefania Raimondo et al.
for 5 min. For the detection of cytotoxicity, the
CellTox™ Green Cytotoxicity Assay
(Catalogue number
G8741
,
Promega
) was used. RAW264.7 and PBMCs were cultured in triplicate into white‐walled, opaque
assay
96‐well
(17)
NAD kinase promotes Staphylococcus aureus pathogenesis by supporting production of virulence factors and protective enzymes
eLife
June 20, 2022
Clarisse Leseigneur, Laurent Boucontet, Magalie Duchateau, Javier Pizarro-Cerda, Mariette Matondo, Emma Colucci-Guyon, Olivier Dussurget
mg/mL) and lysostaphin (0.05 mg/mL) were added. At t=6 hr, 100 μL of the CellTox Green Reagent (
1:500
,
G8741
,
Promega
) were added to
bacteria
. After
incubation
at RT for 15 min, both OD600nm and fluorescent signal
(18)
Nicotinamide‐N‐methyltransferase is a promising metabolic drug target for primary and metastatic clear cell renal cell carcinoma
Clinical and Translational Medicine
June 8, 2022
Anna Reustle et al.
duplicate or triplicate wells. After 24 h, cytotoxicity was assessed by CellTox Green reagent (
Promega
Cat#
G8741
) according to the manufacturer's instructions. Z‐stack images were taken for each well with the
Cytation 1
(19)
O-GlcNAc modification of leucyl-tRNA synthetase 1 integrates leucine and glucose availability to regulate mTORC1 and the metabolic fate of leucine
Nature communications
May 25, 2022
Kim K, Yoo HC, Kim BG, Kim S, Sung Y, Yoon I, Yu YC, Park SJ, Kim JH, Myung K,
et al
.
starvation
, the
cell culture medium
was exchanged with medium containing CellToxTM Green Dye (
Promega
, #
G8741
). Phase and green
fluorescence
images were acquired by using IncuCyteTM Zoom (Essen BioScience). Quantitative
(20)
Extracellular Nucleotides and Histamine suppress TLR3- and RIG-I mediated release of antiviral Interferons from Human Airway Epithelial Cells
The Journal of Immunology
May 15, 2022
Timothy S. Kountz, Assel Biyasheva, Robert P. Schleimer, Murali Prakriya
25% glcyerol, PPIs added fresh. CellTox and CellTiter-Glo
assays
:
CellTox Green Cytotoxicity Assay
(
G8741
) and
CellTiter-Glo 2.0 Assay
(
G9241
) were purchased from
Promega
and manufacturers instructions were
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CellTox Green Cytotoxicity Assay from Promega
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